During this semester I had the opportunity to work, under the supervision of my chairman and the doctoral student wich I am working with, using a UV-VIS scpectrometer and a Fluorimeter. These two are of great importance in my laboratory because it lets us know about the interaction between molecules. In my research project we functionalized the nanoparticles with Ca +2 ions. This was done because the troponin has 4 binding sites of calcium and it can form a complex between the 2 of them. How do we know that they are interacting? when we gathered the results we saw that the absorbance of the nanoparticle alone was a little bit different than the data of absorbance gathered of the nanoparticle that was functionalized letting us know that there was some interaction between them. The Fluorimeter is the same but this time we wanted to measure the level of exitement of the nanoparticle alone and the functionalized nanoparticle. The results were as same as the absorbance, the nanoparticle was a little bit different that the functionalized one letting us know that not only there is an interaction, but at the same time it is a great project because the level of exitement between parlicles is different and it can lead us to find a unique biosensor for the heart attack sensing.
